BEAN-counter is a software pipeline developed within the Myers lab at the University of Minnesota to score genetic
interactions from multiplexed, pooled barcode sequencing experiments. This website serves as a repository for
commonly-used BEAN-counter configuration files and the example dataset used in our recently-submitted protocol.
File | Description | Hypersensitized? (pdr1/pdr3/snq2) |
---|---|---|
Sc_hap_del_wg_hs_v1.txt | S. cerevisiae haploid whole genome gene deletion collection | Yes |
Sc_hap_del_mp_hs_v1.txt | Diagnostic subset of S. cerevisiae haploid whole genome gene deletion collection | Yes |
Sc_HETDIP_hs_essentials_v1.txt | S. cerevisiae essential gene heterozygous diploid deletion collection | Yes |
Sc_TS-essential_hs_v3.txt | S. cerevisiae essential gene haploid temperature-sensitive mutant collection | Yes |
Sc_MoBY_ORF_v1.txt | S. cerevisiae MoBY-ORF gene overexpression collection | No |
Sp_hap_del_wg_v2.txt | S. pombe haploid whole genome gene deletion collection | No |
Ca_del_wg_bc20.txt | C. albicans whole genome homozygous gene deletion collection | No |
File | Description |
---|---|
Sc_hap_MP-WG-HET-MoBY.yaml | For all screens performed with 10bp-indexed forward primers using the barcoding scheme in the S. cerevisiae WG deletion, HETDIP-essential, or MoBY collections |
Sc_hap_TS_ix10.yaml | For all screens performed with 10bp-indexed forward primers using the barcoding scheme in the S. cerevisiae TS-essential collection |
Sp_hap_WG_ix10.yaml | For all screens performed with 10bp-indexed forward primers using the barcoding scheme in the S. pombe WG deletion collection |
Sp_hap_WG_ix8.yaml | For all screens performed with 8bp-indexed forward primers using the barcoding scheme in the S. pombe WG deletion collection |
Ca_del_wg_bc20.yaml | For all screens performed with 10bp-indexed forward primers using the length 20 barcodes in the C. albicans WG deletion collection |
The example dataset is located here.